Qu Ye-hui, Ding Li-jun, Peng Wen-yuan, et al. Optimization of Enzymolysis of Tilapia Waste with Response Surface Methodology by Elastase[J]. 2016, 33(5): 77-82. DOI: 10.3969/j.issn.1007-7162.2016.05.014.
Qu Ye-hui, Ding Li-jun, Peng Wen-yuan, et al. Optimization of Enzymolysis of Tilapia Waste with Response Surface Methodology by Elastase[J]. 2016, 33(5): 77-82. DOI: 10.3969/j.issn.1007-7162.2016.05.014.DOI:
and response surface analysis method is used to optimize the enzymatic processes. The enzyme concentration
temperature
pH
solid to liquid ratio
time and other factors are discussed to analyze the effect of hydrolysis based on the degree of hydrolysis and peptide content. The results show that the optimum conditions of elastase hydrolysis: enzyme concentration 0.553 mg/g
temperature 41.94 ℃
time 3.31 h
pH natural
solid-liquid ratio 1: 5
the degree of hydrolysis and the peptide content is separately 18.09% and 0.90 mg /ml in this condition.