The nucleotide and amino acid sequences of CYP2E1 protein (GenBank accession number: NC000010.11 and NM000773.3) from human being have been analyzed and predicted by bioinformatics in the following aspects: physicochemical properties

hydrophilicity and hydrophobicity

the signal peptide

transmembrane topological structure

subcellular localization

protein secondary structure and tertiary structure

the enzyme active center structure

and the composition of homologous protein and phylogenetic relationship of human being and other 6 species. The results show that the CYP2E1 gene encodes a 493 amino acid polypeptides with the estimated relative mole

cular weight 172 386.6

the theoretical PI of 7.04 and the structural formula of C

6778

H

10986

N

2074

O

2429

S

374

;. It is also found that CYP2E1 is a transmembrane protein with hydrophobicity and relatively unstable. There are 6 β fold

6 α-helix

6 transmembrane regions and 32 angles in its secondary structure

and It's active center was composed of Asn

202

residues

Ser

298

residues

Phe

205

residues and iron porphyrin ring. The distance of its heme and substrate catalytic sites is 0.3-0.6 nm .