两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较

张丽; 谭竹钧; 陈炜; 高珊; 刘牧

doi:10.3969/j.issn.1007-7162.2012.03.016

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两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较

综合研究 | 更新时间：2026-05-27

- 两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较
- Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders
- 广东工业大学学报 2012年29卷第3期页码：81-86
- 作者机构：
- 作者简介：
  
  张丽（1986-）,女，硕士研究生，主要研究方向为低温生物学、大鼠胚胎库.
- 基金信息：
  
  卫生部行业科研专项资金资助项目(20080236)
- DOI：10.3969/j.issn.1007-7162.2012.03.016
  中图分类号：
- 网络首发：2012-09-20，
  
  纸质出版：2012
- 稿件说明：
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张丽, 谭竹钧, 陈炜, 高珊, 刘牧. 两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较[J]. 广东工业大学学报, 2012, 29(3): 81-86.

Zhang Li, Tan Zhu-jun, Chen Wei, et al. Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders[J]. 2012, 29(3): 81-86.
张丽, 谭竹钧, 陈炜, 高珊, 刘牧. 两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较[J]. 广东工业大学学报, 2012, 29(3): 81-86. DOI： 10.3969/j.issn.1007-7162.2012.03.016.

Zhang Li, Tan Zhu-jun, Chen Wei, et al. Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders[J]. 2012, 29(3): 81-86. DOI： 10.3969/j.issn.1007-7162.2012.03.016.

摘要

采用冻存管方法，用 DAP213和SDP0323两种冻存液组合分别对Wistar大鼠2-细胞期胚胎进行玻璃化冷冻，两种冻存液冷冻的胚胎平均复苏率分别为57.1%、69.1%；平均产仔率分别为34.9%、41.1%.SDP0323 冻存液冷冻的胚胎平均复苏率及平均产仔率均比DAP213冻存液冷冻的相对高一些.经统计学分析，两者之间无显著差异（P>0.05）.两组冷冻试剂组合均可作为对 Wistar大鼠2-细胞期胚胎进行冷冻保存的有效方法，可用于建立大鼠胚胎库.

Abstract

The tube method was used to study two different extenders to cryopreserve by vitrification the 2-cell Wistar Rat embryos

which were DAP213 and SDP0323. And the effects of different extenders on the average survival rates of frozen-thawed embryos and on the average rates of the new borns were discussed. The average survival rates and average new born rates after being frozen-thawed were 57.1%

69.1% and 34.9%

41.1% respectively. There are no significant differences between the two extenders using the Student' t-Test

but both the average survival rates and average new born rates with SDP0323 are higher than those with DAP213. The results show that the tube method with DAP213 and SDP0323 for cryopreservation of the 2-cell rat embryos has been successful

and that the two extenders are suitable for the rat 2-cell embryos-bank.

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references

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