生物矿化加固土体方式目前呈现多样化，不同矿化方式又有其各自的局限性，酶诱导碳酸盐沉淀（EICP）缺乏成核位点，而靶向激活产脲酶微生物诱导矿化（生物刺激）加固周期较长、强度较低。针对这些问题，提出生物刺激联合EICP加固土体的矿化方法。以厦门翔安国际机场吹填海砂为试验对象，通过无侧限抗压强度测试（UCS）、碳酸钙生成量测定、扫描电子显微镜（SEM）、X射线衍射（XRD）和微生物多样性检测等试验评价并分析联合方法的加固效果。结果表明：（1）生物刺激联合EICP的矿化方式得到更高的UCS，可以达到2 300 kPa，大于两者单独矿化的强度之和；（2）生物刺激联合EICP的矿化方式可以促进碳酸钙沉淀生成的同时提高其分布均匀性，并优化碳酸钙的晶体结构；（3）微生物的群落分析表明，生物刺激可以高度激活产脲酶细菌，EICP对部分物种有轻微的刺激作用，生物刺激联合EICP的物种丰富程度、均匀程度则介于EICP和生物刺激之间。 Biomineralization of soil solidificationmethods is currently diversified

and different mineralization methods havetheir own limitations. The enzyme-induced carbonateprecipitation (EICP) lacks nucleation sites

while the targeted activationof urease-producing microorganisms induced mineralization (biostimulation) hasa longer solidification cycle and lower strength. Aiming at these problems

amineralization method of biostimulation combined with EICP to reinforce soil isdeveloped. In this paper

reclaimed seasand from Xiamen Xiang'an International Airport was investigated. Unconfined compressive strength tests (UCS)

calciumcarbonate production measurements

scanning electron microscope (SEM)

X-raydiffraction (XRD)

and microbial diversity testing were used to evaluate andanalyze the solidification effect of thecombined method. Some findings were observed. 1) Biostimulation combined with EICPmineralization can get a higher UCS

up to 2 300 kPa

which was greater thanthe sum of the UCS of the two separate mineralizations. 2) Biostimulationcombined with EICP can promote the formation of calcium carbonate precipitationwhile improving its distribution uniformity

and optimize the crystal structureof calcium carbonate. 3) Microbial communityanalysis shows that biostimulation can highly stimulate urease-producingbacteria. EICP has a slight stimulating effect on some species. The species richnessand uniformity of biostimulation combined with EICP are between those of EICPand biostimulation.