Microbial-induced carbonate precipitation is a novel green reinforcement technology in the geotechnical engineering field. To study the reinforcement effect of urease-producing bacteria extracted from the engineering environment in situ

a highly effective urease-producing bacteria was isolated from typical bank slope soil on the Three Gorges Reservoir and identified as Bacillus cereus. To analyze the reinforcement effect

the optimum culture conditions of Bacillus cereus were first determined by orthogonal optimization

and then the solidification tests on a sand column and fractured rock mass were carried out using Bacillus cereus and Sporosarcina pasteurii. The results showed that: (1) Compared with Sporosarcina pasteurii

the porosity of the sand column reinforced by Bacillus cereus was filled more densely

the impermeability was better

and the calcium carbonate engenderment was higher

thus designating that Bacillus cereus had better mineralization function. (2) The permeability coefficient of fractured rock samples reinforced by Bacillus cereus decreased by 3 orders of magnitude

and the internal friction angle and cohesion increased by 48.09% and 85.10% respectively，denoting that Bacillus cereus can efficaciously ameliorate the permeability and shear resistance of fractured rock

and compared with Sporosarcina pasteurii

it has a better reinforcement effect. Relevant research conceptions and results can provide a good reference for the green reinforcement of engineering rock mass.