The seepage prevention and reinforcement of fractured rock mass is one of the key technical problems that affects engineering stability and safety. In this study

the microbial reinforcement technology is applied to grouting reinforcement of fractured rock mass on the basis of the previous technology of microbial reinforcement of sandy soil. The results show that the confining pressure before the reinforcement has a great influence on the hydraulic opening of the fractured rock sample. After the reinforcement

the fracture is filled with calcium carbonate sediments and the seepage of rock sample changes from fissure seepage to pore seepage

and the unit seepage flow and seepage coefficient are controlled by confining pressure and seepage water pressure. After the reinforcement of microbial grouting

the percolation rate per unit time of fractured rock samples decreases by 80.31%-90.04%

and the percolation coefficient can reach the order of 10-6 cm/s. In the process of grouting and reinforcement of fractured rock samples

CaCO3 deposit induced by microorganism during grouting reinforcement of fractured rock samples have good cementation effect

and the splitted rock samples are cemented as a whole to achieve the effect of reinforcement and seepage prevention. The research results can provide a good reference for grouting reinforcement of fractured rock mass.