1. 黑龙江省环境科学研究院
2. 哈尔滨工业大学环境学院
纸质出版:2022
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邢洁, 李婉婷, 许丹妮, 等. 耐钼菌株Serratia marcescens A2的筛选鉴定及其对MoO42-的去除和还原效能[J]. 能源研究与管理, 2022,(4):97-103.
邢洁, 李婉婷, 许丹妮, et al. Isolation and Identification of the Molybdenum-Resistant Strain Serratia marcescens A2 and its Removal and Reduction Efficacy of MoO42-[J]. 2022, (4): 97-103.
邢洁, 李婉婷, 许丹妮, 等. 耐钼菌株Serratia marcescens A2的筛选鉴定及其对MoO42-的去除和还原效能[J]. 能源研究与管理, 2022,(4):97-103. DOI: 10.16056/j.2096-7705.2022.04.016.
邢洁, 李婉婷, 许丹妮, et al. Isolation and Identification of the Molybdenum-Resistant Strain Serratia marcescens A2 and its Removal and Reduction Efficacy of MoO42-[J]. 2022, (4): 97-103. DOI: 10.16056/j.2096-7705.2022.04.016.
大规模的钼矿开采造成了严重的环境污染风险,而微生物对节能高效缓解重金属污染问题具有重要意义。立足环境钼污染的生物修复,通过土壤微生物的富集和筛选得到功能菌株并进行16S rRNA鉴定,分析了筛选菌株对MoO
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的作用效能。实验结果表明筛选得到的菌株为黏质沙雷氏菌(Serratia marcescens A2),其在Luria-Bertani培养基(LB)和低磷酸盐培养基(LPM)中对MoO
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分别表现出去除及还原效能。25℃,pH=7.0时Serratia marcescens A2对MoO
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的去除率最高达到了38.55%,钼在菌株体内的分布及FTIR分析结果表明细胞壁在MoO
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去除过程中发挥着重要作用(占钼去除总量的92.14%)。此外,低温会促进菌株对高浓度MoO
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的去除,而偏酸或偏碱性条件会对菌株的去除及还原效能均产生不利影响。上述结果为钼污染的原位生物修复提供了理论依据。
The amount mining of molybdenum(Mo) has posed significant environmental risks
and microorganisms are of potential for the mitigation of heavy metal pollution energy-savingly and efficiently. Based on the bioremediation of Mo pollution
the functional strains were obtained by enrichment and isolation of soil microorganisms and identified by 16S rRNA. The efficacy of the obt
ained strain on MoO
4
2-
was also analyzed. It was confirmed that the isolated strain was Serratia marcescens A2
which can remove and reduce MoO
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2-
in Luria-Bertani medium(LB) and low phosphate medium(LPM)
respectively. The removal rate of MoO
4
2-
reached 38.55% at 25 ℃ and pH 7.0. The distribution of Mo in Serratia marcescens A2 and the results of FTIR showed that the cell wall performed an essential role in the MoO
4
2-
-removal process(92.14% of the total MoO
4
2-
removed). In addition
a non-neutral environment could seriously inhibit the removal and reduction of MoO
4
2-
by the strain
while the low temperature could promote the removal of MoO
4
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at high concentrations. This study provides a theoretical basis for the in situ microbial remediation of Mo-contaminated environments.
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