石斛多糖提取和对体外黑色素抑制、抗干燥损伤的影响

卫梦尧; 张小辉; 李飞; 吴子锋; 杨永志; 丁金龙

doi:10.12052/gdutxb.210194

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石斛多糖提取和对体外黑色素抑制、抗干燥损伤的影响

更新时间：2026-05-27

- 石斛多糖提取和对体外黑色素抑制、抗干燥损伤的影响
- Extraction of Dendrobium Polysaccharides and Its Effects on Inhibition of Melanin and Resistance to Drying Damage in Vitro
- 广东工业大学学报 2023年40卷第3期页码：91-98
- 作者机构：
  
  广东工业大学生物医药学院,广东,广州,510006
- 作者简介：
  
  卫梦尧(1997–)，女，硕士研究生，主要研究方向为天然药物化学
  丁金龙(1976–)，男，副研究员，博士，主要研究方向为天然药物化学及功能食品研发，E-mail：along31@126.com
- 基金信息：
- DOI：10.12052/gdutxb.210194
  中图分类号： TQ658
- 网络首发：2023-05-25，
  
  纸质出版：2023
- 稿件说明：
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卫梦尧, 张小辉, 李飞, 吴子锋, 杨永志, 丁金龙. 石斛多糖提取和对体外黑色素抑制、抗干燥损伤的影响[J]. 广东工业大学学报, 2023, 40(3): 91-98.

Wei Meng-yao, Zhang Xiao-hui, Li Fei, et al. Extraction of Dendrobium Polysaccharides and Its Effects on Inhibition of Melanin and Resistance to Drying Damage in Vitro[J]. 2023, 40(3): 91-98. DOI: 10.12052/gdutxb.210194.
卫梦尧, 张小辉, 李飞, 吴子锋, 杨永志, 丁金龙. 石斛多糖提取和对体外黑色素抑制、抗干燥损伤的影响[J]. 广东工业大学学报, 2023, 40(3): 91-98. DOI： 10.12052/gdutxb.210194.

Wei Meng-yao, Zhang Xiao-hui, Li Fei, et al. Extraction of Dendrobium Polysaccharides and Its Effects on Inhibition of Melanin and Resistance to Drying Damage in Vitro[J]. 2023, 40(3): 91-98. DOI: 10.12052/gdutxb.210194. DOI：

摘要

用正交试验法优化铁皮石斛多糖的提取工艺条件，并探究了石斛多糖对B16细胞黑色素生成和HaCaT细胞内干燥损伤的影响。通过单因素实验考察了料液比、提取时间、提取次数和提取温度对多糖提取率的影响，进而进行正交试验，优化铁皮石斛多糖提取工艺条件；通过B16细胞实验，测定石斛多糖对细胞增殖率、细胞内酪氨酸酶的活性以及细胞内黑色素含量的影响；通过建立HaCaT细胞干燥损伤模型，测定多糖对细胞干燥损伤的影响。各因素对铁皮石斛多糖提取率影响的顺序为：提取次数料液比提取温度提取时间，最佳提取条件是提取时间为60 min、料液比为1∶20、提取次数2次和提取温度为60 ℃，此工艺下石斛多糖的提取率为12.45%；石斛多糖(10，62.5，125，250，500 μg/mL)对B16细胞活力无影响，在多糖质量浓度为500 μg/mL时，酪氨酸酶活性为69.30%，黑色素含量为66.20%。石斛多糖(10，62.5，125，250，500 μg/mL)对HaCaT细胞活力无影响，在多糖质量浓度为125 μg/mL时，干燥损伤细胞的存活率提高了15.51%，达到了86.58%。本文优选建立了铁皮石斛多糖的最佳提取工艺，并发现石斛多糖可通过抑制细胞内酪氨酸酶活性来减少黑色素含量，建立HaCaT细胞干燥损伤模型，石斛多糖可以提高细胞在干燥损伤中的存活率，减少细胞干燥损伤造成的死亡。

Abstract

In this study

the polysaccharide was extracted by hot water extraction from

Dendrobium officinale

. The polysaccharide extraction rate was used as an indicator. The ratio of extraction material to liquid

the extraction temperature

extraction times and the extraction time were the influencing factors. A single factor experiment was designed. A better extraction process was obtained through orthogonal experiments. The extraction rate of polysaccharides was 12.45%

nd the optimal extraction conditions were: extraction time is 60 min

material-liquid ratio is 1: 20

extraction times are 2 times

and extraction temperature is 60 ℃. The effects of polysaccharides on cell viability

tyrosinase activity and melanin content were determined by B16 cells experiment. By establishing a dry injury model in HaCaT cells

HaCaT cells dry injury model was established

and the cell viability of polysaccharides on cells in dry injury was determined. Different concentrations of polysaccharides (10

62.5

125

250

500 μg/mL) can inhibit melanin production

in the polysaccharide concentration of 500 μg/mL

the tyrosinase activity was 69.30% and the melanin content was 66.20%. Different concentrations of polysaccharides (10

62.5

125

250

500 μg/mL) can reduce cell desiccation damage

and in the polysaccharide concentration of 125 μg/mL

the survival rate of cell desiccation damage increases by 15.51% to 86.58%.

Dendrobium officinale

polysaccharide can reduce the melanin content by inhibiting tyrosinase activity

and can increase the survival rate of cells in desiccation damage and reduce the death of cells caused by desiccation damage. With the establishment of the HaCaT cells desiccation damage model

polysaccharides can improve the survival rate of cells in desiccation damage and reduce the death of cells caused by desiccation damage.

关键词

Keywords

references

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