银耳多糖超声波提取工艺优化及抗BV2细胞炎症的作用研究

谢玲娜; 韩萍; 杜志云

doi:10.12052/gdutxb.200059

欢迎来到能源电力期刊网！

English Version 期刊登录

您当前的位置：

首页 >

文章列表页 >

银耳多糖超声波提取工艺优化及抗BV2细胞炎症的作用研究

综合研究 | 更新时间：2026-05-27

- 银耳多糖超声波提取工艺优化及抗BV2细胞炎症的作用研究
- An Optimization of Ultrasonic Extraction Process of Tremella Polysaccharides and its Anti-inflammation Effect on BV2 cell
- 广东工业大学学报 2021年38卷第2期页码：94-98
- 作者机构：
  
  1. 广东工业大学生物医药学院,广东,广州,510006
  2. 佛山市康伲爱伦生物技术有限公司,广东,佛山,528225
- 作者简介：
  
  谢玲娜(1994–)，女，硕士研究生，主要研究方向为天然药物化学
  杜志云(1969–)，男，教授，研究生导师，主要研究方向为天然药物化学，E-mail：zhiyundu@gdut.edu.cn
- 基金信息：
  
  国家自然科学基金资助项目(21272043)
- DOI：10.12052/gdutxb.200059
  中图分类号： Q946.3
- 网络首发：2021-03-10，
  
  纸质出版：2021
- 稿件说明：
移动端阅览
谢玲娜, 韩萍, 杜志云. 银耳多糖超声波提取工艺优化及抗BV2细胞炎症的作用研究[J]. 广东工业大学学报, 2021, 38(2): 94-98.

Xie Ling-na, Hang Ping, Du Zhi-yun. An Optimization of Ultrasonic Extraction Process of Tremella Polysaccharides and its Anti-inflammation Effect on BV2 cell[J]. 2021, 38(2): 94-98.
谢玲娜, 韩萍, 杜志云. 银耳多糖超声波提取工艺优化及抗BV2细胞炎症的作用研究[J]. 广东工业大学学报, 2021, 38(2): 94-98. DOI： 10.12052/gdutxb.200059.

Xie Ling-na, Hang Ping, Du Zhi-yun. An Optimization of Ultrasonic Extraction Process of Tremella Polysaccharides and its Anti-inflammation Effect on BV2 cell[J]. 2021, 38(2): 94-98. DOI： 10.12052/gdutxb.200059.

摘要

以银耳为原料

采用超声波辅助热水浸提法提取银耳多糖

以多糖提取率为指标

以超声波功率、提取料液比、提取温度和提取时间为影响因素

设计单因素试验

并通过正交试验得到较佳提取工艺。通过用TRPV1(Transient Receptor Potential Vanilloid1)试剂盒检测了小胶质细胞TRPV1的释放水平

与脂多糖(Lipopolysaccharide

LPS)对照组相比

不同质量浓度的银耳多糖组(62.5

250

1000

μg/mL)均能不同程度地抑制TRPV1的释放量。说明了银耳多糖对于BV2小胶质细胞炎症具有良好的抑制作用。

Abstract

In this study

the tremella polysaccharide was extracted by ultrasonic-assisted hot-water extraction from Tremellafuciformis. The polysaccharide extraction rate was used as an indicator. The ultrasonic frequency

the ratio of extraction material to liquid

the extraction temperature

and the extraction time were the influencing factors. A single factor experiment was designed. A better extraction process was obtained through orthogonal experiments. By using the TRPV1 kit to detect the release level of microglial TRPV1

compared with the LPS control group

different concentrations of the Tremella polysaccharide group (62.5

250

1000

μg/mL) can be different to varying degrees Inhibition of TRPV1 release. It shows that Tremella polysaccharide has a good inhibitory effect on BV2 microglia inflammation.

关键词

Keywords

references

WU Y J, WEI Z X, ZHANG F M, et al . Structure, bioactivities and applications of the polysaccharides from tremella fuciformis mushroom: a review [J ] . International Journal of Biological Macromolecul es, 2019, 121: 1005-1010.

LI Y, CAI D L, HU T J, et al . Protective effect of tremella polysaccharides on experimental aging mouse model [J ] . Academic Journal of Second Military Medical University, 2004(10): 1104-1107.

CHEN B. Optimization of extraction of tremella fuciformis polysaccharides and its antioxidant and antitumor activities in vitro [J]. Carbohydrate Polymers, 2010, 81(2): 420-424.

LI H, LEE H S, KIM S H, et al . Antioxidant and anti-inflammatory activities of methanol extracts of tremella fuciformis and its major phenolic acids [J ] . Journal of Food Science, 2014, 79(4): C460-C468.

HANG Z, WANG X, ZHAO M, et al . Free-radical degradation by Fe 2+ /Vc/H 2 O 2 and antioxidant activity of polysaccharide from tremella fuciformis [J ] . Carbohydrate Polymers, 2014, 112: 578-582.

PANG L F. Clinical study about parenteral solution of tremella polysaccharide improving quality of life of patients with liver cancer during chemotherapy [J]. Journal of Hubei University of Chinese Medicine, 2014, 16(4): 85-86.

UKAI S, KIHO T, HARA C, et al . Polysaccharides in fungi. XIV. anti-inflammatory effect of the polysaccharides from the fruit bodies of several fungi [J ] . Pubmed, 1983, 6(12): 983-990.

SHEN T, DUAN C, CHEN B, et al . Tremella fuciformis polysaccharide suppresses hydrogen peroxide-triggered injury of human skin fibroblasts via upregulation of SIRT1 [J ] . Molecular Medicine Reports, 2017, 16(2): 1340-1346.

BACH E E, COSTA S G, OLIVEIRA H A, et al . Use of polysaccharide extracted from tremella fuciformis berk for control diabetes induced in rats [J ] . Thoracic Cardiovascular Surgeon, 2015, 27(7): 2103-2105.

ZHANG X F, GUO Q T, SUN J. Optimization of ultrasonic-assisted extraction process of tremella polysaccharides based on Box-Benhnken response surface methodology [J]. China Pharmacist, 2019(6): 1055-1058.

LIU Q, LIU L, KANG C L, et al . Preparation and research progress of tremella polysaccharides [J ] . Biotechnology Business, 2019(5): 58-63.

DUAN C, WANG C T. Optimization of extracting Tremella fuciformis polysaccharide and detection of its toxicity to cells of human body [J ] . China Surfactant Detergent Cosmetics, 2016, 46282(2): 51-55.

BAO H M. Study on extraction technology and stability of tremella polysaccharide by enzymatic hydrolysis [J]. Food Research and Development, 2015(11): 17-21.

WU Q, ZHENG C, NING Z X, et al . Study on extraction and antioxidant effects of crude alkali-soluble tremella polysaccharide [J ] . Food Science, 2007(6): 153-155.

CHEN Y, ZHAO L, LIU B, et al . Application of response surface methodology to optimize microwave-assisted extraction of polysaccharide from tremella [J ] . Physics Procedia, 2012, 24(part-PA): 429-433.

YANG L, SUI X, QI B, et al . Optimization of ethanol-ultrasound-assisted destabilization of a cream recovered from enzymatic extraction of soybean oil [J ] . Journal of the American Oil Chemists Society, 2019, 91(1): 159-168.

ZHANG W L, HAN Y L. The extraction process of pinus massoniana optimized by response surface [J]. Journal of Guangdong University of Technology, 2013, 30(1): 115-119, 124.

ZHANG Q R, LING L, HE Y Q, et al . Determination of polysaccharide content of blumea balsamifera by phenol sulfuric method [J ] . Chinese Journal of Ethnomedicine and Ethnopharmacy, 2019, 28(14): 30-32.

YANG Y J, JIANG R Z, CHEN Y H, et al . Determination of sugars in heteropolysaccharide by phenol-sulfuric acid method [J ] . Chinese Traditional Patent Medicine, 2005, 27(6): 706-708.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

靶向TSPO的神经炎症成像分子探针

铜钎焊剂的成分优化与制备

多尺度对比嵌入增强的自适应入侵检测模型

基于PSI技术的福州市地面形变监测与影响因素分析

双模态迭代交叉注意力融合集成框架