丁香酚抗马拉色菌活性、细胞毒性及抑菌机制研究

刘迪; 郭清泉; 赵海山; 舒均中; 练英铎; 夏树敏

doi:10.12052/gdutxb.200028

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丁香酚抗马拉色菌活性、细胞毒性及抑菌机制研究

综合研究 | 更新时间：2026-05-27

- 丁香酚抗马拉色菌活性、细胞毒性及抑菌机制研究
- A Study of Eugenol Anti-Malassezia Activity, Cytotoxicity and Antifungal Mechanism
- 广东工业大学学报 2021年38卷第2期页码：88-93
- 作者机构：
  
  1. 广东工业大学轻工化工学院,广东,广州,510006
  2. 广东工业大学生物医药学院,广东,广州,510006
  3. 澳宝化妆品(惠州)有限公司,广东,惠州,516255
- 作者简介：
  
  刘迪(1995–)，女，硕士研究生，主要研究方向为植物去屑止痒剂的开发
  郭清泉(1975–)，男，教授，主要研究方向为天然产物提取应用与特种功能材料制备，E-mail：guoqingquan@gdut.edu.cn
- 基金信息：
  
  国家自然科学基金资助项目(81903600)
- DOI：10.12052/gdutxb.200028
  中图分类号： TQ658.3
- 网络首发：2021-03-10，
  
  纸质出版：2021
- 稿件说明：
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刘迪, 郭清泉, 赵海山, 舒均中, 练英铎, 夏树敏. 丁香酚抗马拉色菌活性、细胞毒性及抑菌机制研究[J]. 广东工业大学学报, 2021, 38(2): 88-93.

Liu Di, Guo Qing-quan, Zhao Hai-Shan, et al. A Study of Eugenol Anti-Malassezia Activity, Cytotoxicity and Antifungal Mechanism[J]. 2021, 38(2): 88-93. DOI: 10.12052/gdutxb.200028.
刘迪, 郭清泉, 赵海山, 舒均中, 练英铎, 夏树敏. 丁香酚抗马拉色菌活性、细胞毒性及抑菌机制研究[J]. 广东工业大学学报, 2021, 38(2): 88-93. DOI： 10.12052/gdutxb.200028.

Liu Di, Guo Qing-quan, Zhao Hai-Shan, et al. A Study of Eugenol Anti-Malassezia Activity, Cytotoxicity and Antifungal Mechanism[J]. 2021, 38(2): 88-93. DOI: 10.12052/gdutxb.200028. DOI：

摘要

用体外药敏试验评价了丁香酚抗马拉色菌的活性

并通过棋盘微量稀释法研究了丁香酚与传统去屑剂联合用药的相互作用

丁香酚的最低抑菌浓度(Minimum Inhibitory Concentration

MIC)为312.50 μg/mL

最低杀菌浓度(Minimum Fungicidal Concentration

MFC)为625.00 μg/mL; 与酮康唑联用表现为协同作用

与吡啶硫酮锌 (Zinc Pyrithione

ZPT)、氯咪巴唑、吡啶酮乙醇胺盐 (Piroctone Olamine

OCT)分别联用表现为相加作用；用四甲基偶唑盐 (3-(4

5-Dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromid，MTT)法评价了丁香酚对角质形成细胞的毒性

其半抑制浓度(50% Inhibitory Concentration，IC

)为320.93 μg/mL

高于传统去屑剂；最后通过扫描电子显微镜 (Scanning Electron Microscope

SEM) 和透射电子显微镜 (Transmission Electron Microscope

TEM) 初步研究了丁香酚对马拉色菌的作用机制

结果表明丁香酚通过破坏细胞壁和细胞内部膜结构达到杀菌的效果。

Abstract

The in vitro susceptibility test was used to evaluate the activity of eugenol against

Malassezia

and the interaction between eugenol and traditional antidandruff drugs was studied by the checkerboard microdilution method. The minimum inhibitory concentration (MIC) of eugenol was 312.50 μg/mL and the minimum fungicidal concentration was 625.00 μg/mL. Eugenol combined with ketoconazole showed a synergistic

effect

and combined with zinc pyrithione (ZPT)

climbazole

and piroctone olamine (OCT) respectively showed additive effects. Combined use can reduce the minimum effective concentration of each drug by 50%~75%. The toxicity of eugenol to keratinocytes was evaluated by MTT (3-(4

5-Dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromid) assay. The results showed that the IC

of eugenol was 320.93 mg/L

which is higher than that of traditional antidandruff agents. This indicated that eugenol has higher safety at the cellular level. The mechanism of eugenol against

Malassezia

was studied by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). It was found that eugenol achieves fungicidal ability by destroying cell wall and internal membrane structure of

Malassezia

. These results provided a theoretical basis for the application of eugenol in antidandruff products.

关键词

Keywords

references

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