Electromagnetic fields（EMF） have been identified as a potential hazard to leukemia. To explore the effects of EMF from walkie talkies on the methylation of Wnt inhibitory factor-1（Wif-1） gene promoter and the proportion of white blood cells

60 male samples based on whether walkie talkies exposed

matched by age

were selected by 1:1 paired design from previous cross-section study. A total of 29 males with and 28 without walkie talkies use were enrolled in final analyses. A questionnaire and physical examination were applied to obtain relevant information. Quantitative DNA methylation analysis was carried out by SEQUENOM assay. Paired Student’s t-test and multivariable linear regression of mixed model were used to explore the associations of walkie talkies with methylation of Wif-1 gene promoter

and the methylation level of Wif-1 with the proportions of white blood cells. Out of 32 CpG sites analyzed in Wif-1 gene promoter

seven sites including CpG7

CpG8

CpG12

CpG16

CpG21

CpG28 and CpG31 had a significant increased level of methylation in the exposure group; and electromagnetic exposure was associated with an increased average methylation level（partial regression coefficent β=2.19

probability P=0.026）. Additionally

the average level of Wif-1 methylation was significantly related with an increased proportion of lymphocytes（β=0.633

P=0.020） while a decreased proportion of eosimophils（β=-0.151

P=0.016）. The present study suggests that electromagnetic fields might raise the proportion of lymphocytes via altering the methylation level of Wif-1 gene promoter. Epigenetic changes may be the important mechanism for the carcinogenic of EMF.