In order to accurately study the distribution of cell transmembrane potential under nanosecond pulses electric field（nsPEF）

we developed a five-layer dielectric model of spherical cell with multi-physics simulation software of COMSOL Multiphysics

and studied the bioelectric effects of dispersion（DP） and electroporation（EP） on a cell with ns PEF. Compared with the results obtained by only taking into account EP

the speed at which the trans-membrane potential（TMP） of point A1 reaches its peak is obviously accelerated; and significant increase in the TMP of B1 during the period from 0 to 100 ns is observed when both DP and EP are introduced; the pore density and TMPs of points A1—A7 with time and along the arc length show that A1—A5 are electroporated successively with A1 at 2 ns

demonstrating that at least 2/3 of the plasma membrane is electroporated. The simulation results demonstrate that accurate prediction of bioelectric effects of nsPEF can be obtained only introducing both DP and EP.