Abstract: Driven by the carbon peak and carbon neutrality goals, the multi-energy coupling relationship of rural agriculture integrated energy system (IES) is more complex. In order to realize reliable operation of agricultural parks, a multi-energy complementary and low-carbon operation optimization scheduling strategy for eco-agriculture IES is proposed. First, considering the energy flow relationship in the agricultural park, the mathematical models of biogas production, multi-energy coupling supply and flexible load demand response are established. Second, considering the uncertainty of photovoltaic, load and biogas, a two-stage robust optimization model of eco-agriculture IES is established. The model introduces carbon emission cost and startup and shutdown cost, which can reduce agricultural carbon emissions and prevent frequent startup and shutdown of units. Then, column and constraint generation algorithm (C&CG) is used to solve the model, combining strong duality theorem and linearization theory. Finally, a numerical example based on IES of an ecological pasture in Jiangxi Province is used to verify the effectiveness of the proposed strategy. The simulation results show that the proposed strategy can realize the coordinated operation of eco-agricultural IES, and improve the system economy, low carbon and energy efficiency.