Abstract: The Problem of climate change caused by the increase of greenhouse gas CO₂ concentration attracts the world’s attention. Stable carbon isotopic characteristics of CO₂ can provide strong support for the study of source and sink mechanism of CO₂. However, this needs the stable carbon isotope composition of different emission sources, specially local or regional sources as data base. The combustion of fossil fuel contributes a lot to the increase of CO₂ concentration, especially the emission of vehicles. In this study, we used laser-based isotope ratio infrared spectrometer（IRIS） to measure the δ¹³C characteristics of CO₂ emitted by gasoline vehicles in China, getting its stable carbon isotope composition（δ 13C） of（-28.0 ± 0.5）‰. Carbon isotope fractionation exists in CO₂ emitted by gasoline vehicles. δ¹³C of CO₂ emitted by gasoline vehicles is not affected by cold start or hot start basically, but the composition of gasoline and engine combustion mode can influence δ¹³C of CO₂. The δ 18 O characteristics of CO₂ emitted by gasoline vehicles changes a lot, with more complicated isotope fractionation. The δ¹³C characteristics of CO₂ from combustion processes of fossil fuels can be a good indicator for fossil fuels. The δ¹³C of CO₂ emitted by gasoline vehicles as representative of China can be obtained from this preliminary study, providing basic foundation for the carbon emissions research in China.