Abstract: Assembly sequence planning（ASP） is one kind of important technology for wind turbine manufacturing. ASP is a combinatorial optimization problem. For wind turbines,the search space as well as the computation of the optimal assembly sequence is huge. To tackle the hard problem,the assembly sequence optimization based on cuckoo algorithm is proposed for wind turbine manufacturing. Firstly,the comprehensive assembly constraints are extracted from the 3D assembly model and represented as four assembly constraint matrices in order to reduce the search space of ASP. Then the assembly sequence objective function is constructed,and the model for assembly sequence planning is established for algorithms to search the optimal assembly sequence. Secondly,the discrete cuckoo algorithm（DCA）is proposed to resolve the ASP model for obtaining the optimal assembly sequence of wind turbines.Finally,the effectiveness of ASP model and excellent performance of DCA are verified by experiments.