Abstract: In order to determine the movement characteristic of raw materials in the anaerobic fermentation reactor under different agitators,the rheological properties of pure chicken manure and mixed raw materials of chicken manure and corn stover with ratio of 1:4（weight basis）under different total solid content were measured. Based on the rheological properties of mixed raw materials,the numerical simulation of four kinds of agitators were analyzed by using CFD. The results show that mixed raw materials is non-Newtonian fluids which is similar to the chicken manure,and its rheological properties can be characterized by power law model. The viscosity of mixed materials decreases with the increasing of siring speed,while the consistency coefficient and rheological index of mixed raw materials are lower than those of chicken manure. The six curved blade agitator has greater influence on the nearby flow field,and the dead zone of the six inclined blade disk turbine agitator is smallest. In addition,the six curved blade agitator has higher radial velocity than the six inclined blade agitator.