Abstract: In order to study the difference of dynamic thermal response between natural ventilation and air conditioning,by comparing the boundary conditions and heat transfer process of the two conditions,the evaluation index of dynamic thermal response of building envelope,Wall Temperature Mode Comprehensive Index,is proposed,which considers the different evaluation connotation of the thermal performance of the wall under the two working conditions. Taking the hot summer and warm winter climate zone as an example,18 wall structures are selected to verify the effectiveness of WTCI in the evaluation of dynamic thermal response of wall under multiple working conditions. The results showed that the index is simple and effective,and can evaluate the thermal response speed of envelope in the process of dynamic heat transfer under multiple working conditions,and can distinguish the performance of heat insulation of wall under different working conditions. The applicability of WTCI in multiple working conditions is analyzed,and the application is suggested.