Abstract: Six sets of high-quality source rocks have been identified globally, with three of them in the pre-Mesozoic strata serving as the primary source rocks for ancient oil and gas reservoirs. Ancient oil and gas reservoirs from the pre-Mesozoic strata exhibit five key characteristics. (1) The predominant basin types include foreland, passive continental margin, and cratonic basins. (2) Their primary type of oil and gas resources remains conventional, although shale oil and gas is developing rapidly. (3) Their oil and gas accumulations are primarily concentrated in the Permian, Devonian, Carboniferous, and Ordovician. (4) Their reservoir lithology is primarily composed of limestones, sandstones, shales, and dolomites. (5) Their burial depth is predominantly within the middle to shallow layers, indicating significant potential for deep plays. The substantial discoveries of ancient oil and gas plays demonstrate enrichment in four fields: the periphery of cratons, carbonate reservoirs, shale oil and shale gas reservoirs, and basement reservoirs. After analyzing the major discoveries in key areas, it is revealed that high-quality source-reservoir-seal combinations form readily in the peripheral regions of cratons that were historically located within low-latitude intertropical convergence zones. Global significant events have played a crucial role in shaping the development of source rocks and the enrichment of shale oil and gas. Within the temporal framework of these significant global events, potential plays can be optimized in advance by reconstructing the paleo-positions of accumulation elements. Based on independent evaluations of recoverable oil and gas reserves and yet-to-be-discovered resources, it is evident that conventional oil and gas exploration should focus on the Arabian Basin, Zagros Basin, Tarim Basin, and other basins. Basement rocks and residual strata are also important potential exploration areas. For shale oil and shale gas exploration, the focus should be on the Devonian Domanik shale in the Timan-Pechora and the Volga-Ural basins in Russia, the Silurian hot shale in the Arabian Basin in the Middle East, the Silurian and Devonian plays in the Ghadames Basin in the North Africa, and several sets of shales in the Sichuan and Junggar basins in China.