Abstract: This paper explores the critical technologies in the design and construction process of the jacking force estimation and relay room setting. The comparison between the measured and estimated jacking force, and the setting and use of relay room are also studied. Based on the pipe jacking project of a 220 kV cable line project crossing the Monkey Island waterway in Zhuhai, the ultra-long distance pipe jacking project involves a single jacking of 1022 m, and uses reinforced concrete pipes with an inner diameter of 2.2 m. The crossing stratum includes clay, mucky soil, coarse sand, etc. Firstly, the jacking force is estimated and the relay room is set according to the specifications. Then, during the implementation of the project, the variation of the jacking force is measured. Finally, the comparison curve between the estimated jacking force and the measured jacking force is obtained. The variation curve of the measured average friction is inversely calculated. The comparative analysis between theoretical calculation and measured results shows that under normal jacking conditions, the average frictional resistance in the whole process can be reduced to about 1.5 kPa. However, after the jacking has been stopped for 48 hours, when starting again, the average frictional resistance in the whole process increased sharply to the level of 4.1 kPa. The measured total jacking force is close to the estimated jacking force value, indicating that using thixotropic mud to reduce resistance and continuous jacking is an ideal state for ultra-long distance pipe jacking. This paper proposes that jacking force control is the key to the success of ultra-long distance pipe jacking, and it is suggested to take the average frictrional resistance value according to the stratum section, and the configuration scheme of relay room is proposed.