Bioinformatic Analysis of the Main Characteristics and Structure of the Gene Encoding CYP2E1 Protein

Lin Fei-fan; Han Ya-li

doi:10.3969/j.issn.1007-7162.2015.01.004

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Bioinformatic Analysis of the Main Characteristics and Structure of the Gene Encoding CYP2E1 Protein

更新时间：2026-05-27

- Bioinformatic Analysis of the Main Characteristics and Structure of the Gene Encoding CYP2E1 Protein
- Vol. 32, Issue 1, Pages: 16-19(2015)
- 作者机构：
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- DOI：10.3969/j.issn.1007-7162.2015.01.004
  CLC：
- Online First：05 March 2015，
  
  Published：2015
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林非凡, 韩雅莉. 人CYP2E1基因编码蛋白主要特性与结构的生物信息学分析[J]. 广东工业大学学报, 2015, 32(1): 16-19.

Lin Fei-fan, Han Ya-li. Bioinformatic Analysis of the Main Characteristics and Structure of the Gene Encoding CYP2E1 Protein[J]. 2015, 32(1): 16-19.
林非凡, 韩雅莉. 人CYP2E1基因编码蛋白主要特性与结构的生物信息学分析[J]. 广东工业大学学报, 2015, 32(1): 16-19. DOI： 10.3969/j.issn.1007-7162.2015.01.004.

Lin Fei-fan, Han Ya-li. Bioinformatic Analysis of the Main Characteristics and Structure of the Gene Encoding CYP2E1 Protein[J]. 2015, 32(1): 16-19. DOI： 10.3969/j.issn.1007-7162.2015.01.004.

摘要

采用生物信息学方法对人CYP2E1基因编码蛋白质的结构、理化性质、亲疏水性、信号肽、跨膜结构域、亚细胞定位、二级结构、三级结构、酶活性中心等进行预测，并对人和其他6物种的CYP2E1编码蛋白序列进行系统进化分析.研究结果表明，人CYP2E1编码493个氨基酸组成多肽，其相对分子质量约为172 386.6

等电点理论值为7.04，分子式为C

6778

10986

2074

2429

374

.该蛋白定位于细胞膜外，属跨膜蛋白，为疏水性不稳定蛋白；该蛋白二级结构有6个β折叠、6个α-螺旋、6个跨膜结构区和32个转角，该酶活性中心由Asn

202

残基、Ser

298

残基及Phe

205

残基与铁卟啉环构成，CYP2E1蛋白血红素铁和底物催化位点之间的距离在0.3~0.6 nm之间.

Abstract

The nucleotide and amino acid sequences of CYP2E1 protein (GenBank accession number: NC000010.11 and NM000773.3) from human being have been analyzed and predicted by bioinformatics in the following aspects: physicochemical properties

hydrophilicity and hydrophobicity

the signal peptide

transmembrane topological structure

subcellular localization

protein secondary structure and tertiary structure

the enzyme active center structure

and the composition of homologous protein and phylogenetic relationship of human being and other 6 species. The results show that the CYP2E1 gene encodes a 493 amino acid polypeptides with the estimated relative mole

cular weight 172 386.6

the theoretical PI of 7.04 and the structural formula of C

6778

10986

2074

2429

374

;. It is also found that CYP2E1 is a transmembrane protein with hydrophobicity and relatively unstable. There are 6 β fold

6 α-helix

6 transmembrane regions and 32 angles in its secondary structure

and It's active center was composed of Asn

202

residues

Ser

298

residues

Phe

205

residues and iron porphyrin ring. The distance of its heme and substrate catalytic sites is 0.3-0.6 nm .

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