Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders

Zhang Li; Tan Zhu-jun; Chen Wei; Gao Shan; Liu Mu

doi:10.3969/j.issn.1007-7162.2012.03.016

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Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders

更新时间：2026-05-27

- Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders
- Vol. 29, Issue 3, Pages: 81-86(2012)
- 作者机构：
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- DOI：10.3969/j.issn.1007-7162.2012.03.016
  CLC：
- Online First：20 September 2012，
  
  Published：2012
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张丽, 谭竹钧, 陈炜, 高珊, 刘牧. 两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较[J]. 广东工业大学学报, 2012, 29(3): 81-86.

Zhang Li, Tan Zhu-jun, Chen Wei, et al. Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders[J]. 2012, 29(3): 81-86.
张丽, 谭竹钧, 陈炜, 高珊, 刘牧. 两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较[J]. 广东工业大学学报, 2012, 29(3): 81-86. DOI： 10.3969/j.issn.1007-7162.2012.03.016.

Zhang Li, Tan Zhu-jun, Chen Wei, et al. Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders[J]. 2012, 29(3): 81-86. DOI： 10.3969/j.issn.1007-7162.2012.03.016.

摘要

采用冻存管方法，用 DAP213和SDP0323两种冻存液组合分别对Wistar大鼠2-细胞期胚胎进行玻璃化冷冻，两种冻存液冷冻的胚胎平均复苏率分别为57.1%、69.1%；平均产仔率分别为34.9%、41.1%.SDP0323 冻存液冷冻的胚胎平均复苏率及平均产仔率均比DAP213冻存液冷冻的相对高一些.经统计学分析，两者之间无显著差异（P>0.05）.两组冷冻试剂组合均可作为对 Wistar大鼠2-细胞期胚胎进行冷冻保存的有效方法，可用于建立大鼠胚胎库.

Abstract

The tube method was used to study two different extenders to cryopreserve by vitrification the 2-cell Wistar Rat embryos

which were DAP213 and SDP0323. And the effects of different extenders on the average survival rates of frozen-thawed embryos and on the average rates of the new borns were discussed. The average survival rates and average new born rates after being frozen-thawed were 57.1%

69.1% and 34.9%

41.1% respectively. There are no significant differences between the two extenders using the Student' t-Test

but both the average survival rates and average new born rates with SDP0323 are higher than those with DAP213. The results show that the tube method with DAP213 and SDP0323 for cryopreservation of the 2-cell rat embryos has been successful

and that the two extenders are suitable for the rat 2-cell embryos-bank.

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references

徐平.实验动物胚胎和合子的低温保存[J].中国实验动物学杂,2000,10(4): 240-244.

Mizuno A,Hoshi M,Hirabayashi M. Cryopreservation of SHR embryos [J]. ProcJpnSoc Anim Models HumDis,1987,3:42-45.

Rall W F,Fahy G M. Ice-free cryopreservation of mouse embryo at -196 ℃ by vitrification. [J]. Nature,1985, 31（3）: 573-575.

中泻直己.超急速冻结法を用いた体外受精由来マウス& 细胞期胚の冻结保存について[J].日本不妊学会杂志,1989,34:470-474.

Hirabayashi M, Takahashi R, Sekiquchi J, et al. Viability of transgenic rat embryos after freezing and thawing [J]. Exp Anim,1997,46(2):111-115.

Whittingham D G. Survival of rat embryos after freezing and thawing [J].J Reprod Fertil, 1975,43: 575-578.

Naomi Nakagata.Studies on cryopreservation of embryos and gameted in mice [J]. Exp Anim,1995, 44(1)：1-8.

Kazuki Nakao, Naomi Nakagata, Motoya Katsuki.Simple and efficient vitrification procedure for cryopreservation of mouse embryos[J].Exp Anim, 1997, 46(3)：231-234.

周生来, 于洋, 王维，等. SD 大鼠超排卵及胚胎冷冻方法比较[J]. 实验动物与比较医学,2008,28(6):397-401.

Goh V H H, Adiga S K, Tain C F,et al. Successful in vitro growth of rat two-cell embryos to blastocysts using a simple chemically defined medium[J]. Journal of Pharmacological and Toxicological Methods,2000, 43:171-175.

左琴, 岳秉飞, 刘双环，等. Wistar大鼠超数排卵及胚胎移植 [J].实验动物科学与管理,2003, 20:70-72.

Dinnyes A,Wallace G,Rall W.Effect of genotype on the efficiency of mouse embryo cryopreservation by vitrification or slow freezing method[J]. Molecular Reproduction and development. 1995,40:429-435.

Pomp D, Eisen E J. Gentic control of survival of frozen mouse embryos [J]．Biol Reprod. 1990, 42:775-786.

Han M S, Niwa K. Vitrification of rat embryos at various developmental stages[J]. Theriogenology,2003,59(8):1851-1863.

梁成光,崔建英,薛晓先,等.利用不同冷冻方法建立大鼠胚胎保种库研究[J].内蒙古大学学报,2001,32（6）:653-656.

Kasai M. Vitrification: refined strategy for the cryopreservation of mammalian embryos[J].Mamm OvaRes,1997,14:17-28.

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