A Study of Bacterium Growth Curve and Suspended Matter in the Medium

Du Qing-ping; Wang Qian; Cao Li-chuang; Zhang Xiao-tong; Zeng Qi-hao; Deng Tong-chu

doi:10.3969/j.issn.1007-7162.2012.03.015

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A Study of Bacterium Growth Curve and Suspended Matter in the Medium

更新时间：2026-05-27

- A Study of Bacterium Growth Curve and Suspended Matter in the Medium
- Vol. 29, Issue 3, Pages: 77-80(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-7162.2012.03.015
  CLC：
- Online First：20 September 2012，
  
  Published：2012
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杜青平, 王倩, 曹立创, 张晓彤, 曾起豪, 邓通初. 悬浮物变化引起细菌JS17生长曲线的波动规律[J]. 广东工业大学学报, 2012, 29(3): 77-80.

Du Qing-ping, Wang Qian, Cao Li-chuang, et al. A Study of Bacterium Growth Curve and Suspended Matter in the Medium[J]. 2012, 29(3): 77-80.
杜青平, 王倩, 曹立创, 张晓彤, 曾起豪, 邓通初. 悬浮物变化引起细菌JS17生长曲线的波动规律[J]. 广东工业大学学报, 2012, 29(3): 77-80. DOI： 10.3969/j.issn.1007-7162.2012.03.015.

Du Qing-ping, Wang Qian, Cao Li-chuang, et al. A Study of Bacterium Growth Curve and Suspended Matter in the Medium[J]. 2012, 29(3): 77-80. DOI： 10.3969/j.issn.1007-7162.2012.03.015.

摘要

为了探索一株细菌JS17的生长特性，同时采用活菌计数法和分光光度法测定JS17的生长曲线，并对两种结果进行对比分析.活菌计数法能够真实可靠地反映体系中的活菌数量，但操作繁琐，要求高，耗时长；比浊法方便快捷，但测得的菌体浓度受死亡菌体及其它悬浮物的影响较大.从生长曲线得出JS17在松醇油液体培养基中摇床培养时，0~20 h为生长延滞期，20~48 h为指数生长期，48~72 h为生长稳定期，72 h之后为衰亡期.结合两种方法对细菌生长规律进行测定，就可以较为全面地反映细菌的生长繁殖以及悬浮物代谢变化的规律.

Abstract

The bacterium growth curve was determined via the two methods

surviving bacteria count and spectrophotometry

used to study the growth characteristics of a bacterium called JS17. The results obtained by means of the surviving bacteria count can reflect the actual number of live bacteria，but this method needs many operations and it is time-consuming. It is more convenient to use the spectrophotometry method

but the results got by means of this method were influenced by other suspended matter

which were possibly the chemical reaction materials or metabolism products of JS17. The growth curve of JS17

with shaking culture in 2# oil medium

shows that lag phase in 0~20 h，log phase in 20~48 h

stationary phase in 48~72 h and decline phase emerge after 72 h. The results suggest that the suspended matter affects the rule of growth curve. The metabolism of the suspended matter of bacterium JS17 deserves further study.

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references

张艳，戴晶平．凡口铅锌矿选矿废水资源化研究与应用[J]．有色金属:选矿部分，2007(6): 33-35.

栾和林，姚文．矿山化学药剂污染及其复合污染问题与探讨[J]．矿冶，2002，11（7）：265-267.

Gao Ying, Chu Wei-wei, Zhang Xia, et al. Isolation and determination of growth curve of deoderizing microorganism from swine manure[J]. Journal of Mountain Agriculture and Biology，2011，30 (1)： 12-14.

代群威，李琼芳．环境工程微生物学实验[M]．北京：化学工业出版社，2009:12-15.

李庆忠，张忠智，王洪君，等．一株代谢煤油兼性厌氧菌的筛选鉴定和特性[J]．微生物学通报，2002，29（3）：134-135.

李志恒，王廷英，朱正祥,等．解吸水解废液中微量尿素分析方法讨论[J]．化肥设计，2011，49(2)：31-33.

熊海燕，李莹．不同果汁发酵液中酵母菌生长曲线的测定及pH值的变化[J]．农产品加工学刊，2009(4)：26-27.

李华，张世华，班春兰．烟气脱硫菌株生长曲线的测定及生理生化试验[J]．洁净煤技术，2007,13(1)：44-45.

辛华，马武．现代细胞生物学技术[M]．北京：科学出版社，2009: 142-143.

周德庆．微生物学教程[M]．北京：高等教育出版社，2010: 156-157.

刘婕，杨博．一株高效油脂降解菌的分离鉴定及其性能研究[J]．中国油脂，2010，35(1)：41-43.

Zhang Guo-liang, Wu Yue-ting, Qian Xin-ping, et al. Biodegradation of crude oil by pseudomonas aeruginosa in the presence of rhamnolipids[J]．Journal of Zhejiang University，2005，6(8)：725-730.

R E.布坎南，N E.吉本斯.伯杰细菌鉴定手册[M].8版．北京：科学出版社，1984.

熊宇迪，刘霞，冯长根．杂多酸(蓝)分光光度法在测定痕量元素中的研究进展[J]．光谱学与光谱分析，2008，28(1)：231-232.

Anthony Arnoldo, Jasna Curak, Kittanakom Saranya，et al. Identification of small molecule inhibitors of pseudomonas aeruginosa exoenzyme S using a yeast phenotypic screen[J]．PLoS Genet,2008，4(2)：346-327．

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