Protective Effect of Schisandra Chinensis Oil on H2O2-induced Oxidative Stress Injury in HaCaT Cells by 3D Skin Model

Tai Mei-ling; Jiang Ling; Li Yuan-zhao; Han Ping; Lin Li; Du Zhi-yun

doi:10.12052/gdutxb.210155

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Protective Effect of Schisandra Chinensis Oil on H₂O₂-induced Oxidative Stress Injury in HaCaT Cells by 3D Skin Model

更新时间：2026-05-27

- Protective Effect of Schisandra Chinensis Oil on H₂O₂-induced Oxidative Stress Injury in HaCaT Cells by 3D Skin Model
- Vol. 40, Issue 1, Pages: 130-136(2023)
- 作者机构：
  
  1. 无限极(中国)有限公司,广东,江门,529000
  2. 广东工业大学生物医药学院,广东,广州,510006
  3. 佛山市康伲爱伦生物技术有限公司,广东,佛山,528231
- 作者简介：
- 基金信息：
- DOI：10.12052/gdutxb.210155
  CLC： R965.1
- Online First：25 January 2023，
  
  Published：2023
- 稿件说明：
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太美灵, 江岭, 李宛钊, 韩萍, 林丽, 杜志云. 3D皮肤模型法评价五味子油对H₂O₂诱导的HaCaT细胞氧化损伤的保护作用[J]. 广东工业大学学报, 2023, 40(1): 130-136. DOI: 10.12052/gdutxb.210155.

Tai Mei-ling, Jiang Ling, Li Yuan-zhao, et al. Protective Effect of Schisandra Chinensis Oil on H₂O₂-induced Oxidative Stress Injury in HaCaT Cells by 3D Skin Model[J]. 2023, 40(1): 130-136. DOI: 10.12052/gdutxb.210155.
太美灵, 江岭, 李宛钊, 韩萍, 林丽, 杜志云. 3D皮肤模型法评价五味子油对H₂O₂诱导的HaCaT细胞氧化损伤的保护作用[J]. 广东工业大学学报, 2023, 40(1): 130-136. DOI: 10.12052/gdutxb.210155. DOI：

Tai Mei-ling, Jiang Ling, Li Yuan-zhao, et al. Protective Effect of Schisandra Chinensis Oil on H₂O₂-induced Oxidative Stress Injury in HaCaT Cells by 3D Skin Model[J]. 2023, 40(1): 130-136. DOI: 10.12052/gdutxb.210155. DOI：

摘要

运用3D皮肤模型法评价五味子油对H

诱导的HaCaT细胞氧化损伤的保护作用。利用水蒸气蒸馏法提取得到五味子油

采用GC-MS分析其化学成分

通过DPPH、ABTS自由基清除实验

以及H

诱导HaCaT细胞损伤2D、3D皮肤模型评价五味子油的抗氧化作用。结果表明

五味子油中主要化学成分包括依兰烯、

-喜马烯、

-蒎烯等37种化合物

五味子油对DPPH、ABTS自由基具有良好的清除效果

其清除率的IC

分别为5.6 mg/mL、9.4 mg/mL; 五味子油能提高H

处理后的HaCaT细胞存活率

增加3D皮肤模型的表皮层厚度

降低白细胞介素-6 (Interleukin-6

IL-6)水平

上调SOD酶和CAT酶活性。研究结果证实

五味子油对H

诱导的HaCaT细胞氧化应激损伤模型具有保护作用

可为五味子油皮肤抗衰老产品的研发奠定基础。

Abstract

The protective effect of

Schisandra chinensis

oil on H

-induced oxidative damage of HaCaT cells is evaluated by 3D skin model.

Schisandra chinensis

oil was extracted by steam distillation

and its chemical constituents analyzed by GC-MS. And the antioxidant activity of

schisandra chinensis

oil was evaluated by DPPH and ABTS free radical scavenging experiments

combined with 2D and 3D skin models ind

uced by H

. The results showed that

Schisandra chinensis

oil mainly contained 37 compounds including ylenene

-cimarene and

-pinene.

Schisandra chinensis

oil had good scavenging effect on DPPH and ABTS free radicals

with IC

of 5.6 mg/mL and 9.4 mg/mL

respectively. What’s more

Schisandra chinensis

oil can improve the survival rate of HaCaT cells after H

treatment

increasing the epidermal thickness of 3D skin model

decreasing the level of Interleukin-6(IL-6)

and up-regulating the activities of SOD and CAT enzymes. The results confirmed that

Schisandra chinensis

oil had protective effect on H

-induced oxidative stress injury model of HaCaT cells

which could lay a foundation for the development of anti-aging products of

schisandra chinensis

oil.

关键词

Keywords

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