In order to investigate the effect of treated objects on atmospheric pressure plasma jet characteristics during its application for skin wound healing

we investigated the basic optical-electrical characteristics

the processes of generation and evolution

and the emission characters of spectrum of the argon APPJ under the conditions of free standing jet state and of interacting with culture medium and skin tissue. The experimental results show that

compared with those in the free standing jet state

the length and diameter of APPJ outside nozzle are increased when Ar APPJ interacts with the culture medium and skin tissue. Besides

the excited particles and reactive species at different positions in the axial direction are basically the same as those in the free standing jet state

only the relative intensity of spectral lines is different. Moreover

the relative intensities of OH radical

the second positive band system of the nitrogen molecule

and the excited state Ar and O atom in the place 15 mm away from nozzle will increase compared with those in the free standing jet state. The numerical simulation results of electric and flow field distribution indicate that

compared with in the free standing jet state

the treated objects with different permittivity and state set outside the quartz tube not only make the applied electric field intensity to be strengthened

but also change the molar concentration distribution of working gas outside the tube.These factors may further change the optical and electrical characteristics of Ar APPJ eventually.