Abstract: The integration of high proportional renewable power generation has brought great challenges to the power system frequency stability and put forward a new requirement for the power system safe and economical operation. Most of the existing studies use the integration of frequency stability constraints to solve this problem. However, this treatment normally needs the linearization process or the complex non-linear optimization to obtain the solution. Also, these studies usually deal with the problem of insufficient inertia with scheduling. Inspired by the minimum inertia evaluation, this paper proposes an optimal operation for the power system with a high proportion of renewable power generation. Based on a three-step operational framework of "pre-treatment"-" minimum inertia evaluation"-"optimal operation", the problem of power system optimal operation integrated with the frequency stability constraint is decoupled into two steps with "minimum inertia evaluation" and "optimal operation". In this proposed method, the frequency stability of the system is ensured by coupling the minimum power system inertia constraint. In addition, the support of inertia by ways of scheduling and virtual inertia improvement is also considered in this paper. The proposed method has the advantages of clear planning and easy implementation with a high degree of linearization, having practical application prospects. Finally, the effectiveness and superiority of the method is verified by simulation analysis.