Abstract: On the background of in-depth reform of the electricity market, integrated energy service providers are not only the main energy providers directly providing energy services for users, but also an important carrier of energy conservation and emission reduction. Research on the cooperative operation of integrated energy service providers will help to promote the low-carbon economy of regional integrated energy systems. Firstly, this paper proposes the regulatory framework of two-layer collaborative optimization operation, which mobilizes the enthusiasm of comprehensive energy service providers at the bottom layer to participate in the carbon emission reduction through top-layer supervision, and the stakeholders at the bottom layer with limited information to achieve collaborative optimization. Secondly, based on Nash bargaining theory, a cooperative game model of integrated energy service providers considering carbon emission limits and penalty coefficients is established in order to explore the potential of cooperative operation of integrated energy service providers to promote carbon emission reduction and improve individual interests and social benefits. Then, the non-convex model is decomposed into two convex optimization problems under linear constraints, namely the benefit maximization sub-problem and the energy transaction payment sub-problem. The adaptive alternating direction method of multipliers is further used to solve the above problems. Finally, the effectiveness of the proposed strategy is verified by examples.