Abstract: In order to study the influence of acoustic wave characteristics on fluid flow, an experimental platform was built based on which, the research for factors affecting on flow velocity such as acoustic wave frequency, sound pressure level（SPL）, etc. In addition, sound and flow fields were numerically calculated via the Comsol software, and the errors between the experimental results and the calculated data were analyzed accordingly. Results show that when SPL is less than 125 dB, the flow velocity is not affected by the sound wave either in the same or opposite direction; when SPL lies in 125-＜132 dB and the acoustic wave is in the same direction with fluid, the variation of the fluid velocity pulsation amplitude is weakened and the laminar flow state is strengthened with the increase of SPL; when SPL is 132-155 dB, the fluid velocity pulsation amplitude increases with the increase of SPL, and it would have an explosive increment and lead to turbulence shock when SPL reaches about 153 dB. Moreover, when the acoustic wave is in the opposite direction with fluid, the fluid velocity pulsation amplitude firstly increases and then changes slowly with the increase of SPL which lies in 125-＜145 dB; when SPL is 145-155 dB, the fluid velocity pulsation amplitude also increases with SPL rising;, the fluid velocity pulsation amplitude has a slow variation with the increase of the sound wave frequency under the same SPL. The sound wave frequency has no effects on fluid velocity when the acoustic wave is in the same direction with fluid, and the growth rate of the fluid velocity pulsation amplitude is higher than that when the acoustic wave is opposite to the fluid.