Abstract: A variety of factors affecting the safety culture evaluation index system of power generation enterprises are analyzed, based on which a safety culture evaluation index system of power generation enterprises is established. The analytic hierarchy process（AHP） is used to construct a judgment matrix, calculate the weight of single layer indicators, sort the indicators between levels, and determine the weight of all indicators relative to the total indicators. The MATLAB software is used to write the corresponding programs, effectively reducing the burden of calculations. The case study shows that this method enables to evaluate the safety culture of power generation enterprises scientifically and effectively, with a strong operability, which is of significance for guiding the construction of safety culture of power generation enterprises.